|
|
Voltammetric detection of 7α-hydroxycholesterol on boron doped diamond electrode
Serbanová, Lucie ; Schwarzová, Karolina (advisor) ; Nesměrák, Karel (referee)
This bachelor's thesis is dedicated to the electrochemical determination of 7α-hydroxycholesterol (7α-OHC) using boron-doped diamond electrode (BDDE). The work includes the optimization of experimental conditions and the measurement of the concentration dependence of 7α-OHC. The influence of various factors, such as water content in the solution and scan rate, on the electrochemical response of 7α-OHC was investigated. Various electrochemical methods, including cyclic voltammetry and differential pulse voltammetry, were used in the experiments. The electrochemical behavior of 7α-OHC in a solution of chloric acid and acetonitrile were analyzed. Important factors such as electrode passivation and response stability were carefully evaluated. The measurement of concentration dependence of 7α-OHC exhibited a linear response within the concentration range from 2∙10−5 mol l−1 to 1∙10−4 mol l−1 . Limits of detection (LOD) and quantification (LOQ) of 1,33 µmol l−1 and 4,43 µmol l−1 were determined. Comparison with other methods revealed that the electrochemical determination of 7α-OHC on BDDE achieves comparable sensitivity. The results of this work provide crucial insights into the electrochemical behavior of 7α-OHC, highlighting the advantages of BDDE for sensitive and reliable determination of...
|
|
|
Gene expression study of oxysterol signal pathway in breast cancer patients
Kloudová, Alžběta ; Souček, Pavel (advisor) ; Vopálenský, Václav (referee)
Hormonal therapy is a common part of breast carcinoma treatment in patients whose tumors express estrogen and progesterone receptors. The aim of hormonal therapy is to prevent proliferative effect of hormones througt their receptor proteins in order to inhibit tumor growth. However, certain number of tumors is resistant to hormonal therapy despite expression of hormonal receptors. Presently, the reasons of this resistance are not fully understood. Oxysterols are hydroxylated cholesterol derivates, which may play some role in development of the resistance. They may interfere with hormonal therapy effect and influence some signal pathways leading to cancer progression. This study comes with results of gene expression of proteins influenced by oxysterol action, metabolic and transport proteins, transcription factors and members of signaling pathways that may be related to oxysterol effect. This thesis identifies some candidate genes for future analysis on the basis of comparison of gene expression between estrogen receptor positive and negative tumors and correlation with clinopathological data. The final goal should lead to discovery of new diagnostic markers for breast cancer therapy. Powered by TCPDF (www.tcpdf.org)
|
|
|
Gene expression study of oxysterol signal pathway in breast cancer patients
Kloudová, Alžběta ; Souček, Pavel (advisor) ; Vopálenský, Václav (referee)
Hormonal therapy is a common part of breast carcinoma treatment in patients whose tumors express estrogen and progesterone receptors. The aim of hormonal therapy is to prevent proliferative effect of hormones througt their receptor proteins in order to inhibit tumor growth. However, certain number of tumors is resistant to hormonal therapy despite expression of hormonal receptors. Presently, the reasons of this resistance are not fully understood. Oxysterols are hydroxylated cholesterol derivates, which may play some role in development of the resistance. They may interfere with hormonal therapy effect and influence some signal pathways leading to cancer progression. This study comes with results of gene expression of proteins influenced by oxysterol action, metabolic and transport proteins, transcription factors and members of signaling pathways that may be related to oxysterol effect. This thesis identifies some candidate genes for future analysis on the basis of comparison of gene expression between estrogen receptor positive and negative tumors and correlation with clinopathological data. The final goal should lead to discovery of new diagnostic markers for breast cancer therapy. Powered by TCPDF (www.tcpdf.org)
|
guest ::
